Quantitative PCR for hepatitis B virus with colorimetric detection.
نویسندگان
چکیده
منابع مشابه
Quantitative PCR for hepatitis B virus with colorimetric detection.
A novel, sensitive colorimetric test is described for quantification of the initial number of hepatitis B virus (HBV) genomes amplified in PCR. The viral genomes are amplified together with a synthetic internal standard (IS) to correct for the variability of the efficiency factor. One of the two primers is biotinylated, and the amplified mixtures of HBV and IS DNAs are bound to streptavidin-coa...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1993
ISSN: 1088-9051
DOI: 10.1101/gr.3.3.169